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α ugcg  (Bioss)
94
Bioss α ugcg
Cells do not compensate for the lack of GLTP by increasing levels of <t>UGCG</t> or LTPs, although protein localizations are altered in the absence of GLTP . A , Western blot of analysis of the levels of UGCG, FAPP2 and CERT in WT HeLa, GLTP KO, GLTP rescue and ΔFFAT-GLTP HeLa cells. Antibodies used are described in the Materials section. Beta-actin was used as a loading control. The protein band in the upper blot that runs between FAPP2, and beta-actin is unidentified. B , representative fluorescence images of coimmunostaining of glucosylceramide synthase UGCG ( green ) and Golgi marker golgin-97 ( red ) in HeLa WT cells and, ( C ) GLTP KO HeLa cells. D , immunostaining of UGCG ( green ) and golgin-97 ( red ) in GLTP rescue HeLa cells and ( E ) ΔFFAT-GLTP expressing HeLa cells. The scale bar represents 200 μm. The subpanels are higher magnifications (2x) of representative cells. CERT, ceramide transport protein; FAPP2, four-phosphate adaptor protein 2; UGCG, UDP-glucose ceramide glucosyltransferase; GLTP, glycolipid transfer protein; GLTP KO, GLTP knockout.
α Ugcg, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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860506p  (Croda International Plc)
91
Croda International Plc 860506p
Cells do not compensate for the lack of GLTP by increasing levels of <t>UGCG</t> or LTPs, although protein localizations are altered in the absence of GLTP . A , Western blot of analysis of the levels of UGCG, FAPP2 and CERT in WT HeLa, GLTP KO, GLTP rescue and ΔFFAT-GLTP HeLa cells. Antibodies used are described in the Materials section. Beta-actin was used as a loading control. The protein band in the upper blot that runs between FAPP2, and beta-actin is unidentified. B , representative fluorescence images of coimmunostaining of glucosylceramide synthase UGCG ( green ) and Golgi marker golgin-97 ( red ) in HeLa WT cells and, ( C ) GLTP KO HeLa cells. D , immunostaining of UGCG ( green ) and golgin-97 ( red ) in GLTP rescue HeLa cells and ( E ) ΔFFAT-GLTP expressing HeLa cells. The scale bar represents 200 μm. The subpanels are higher magnifications (2x) of representative cells. CERT, ceramide transport protein; FAPP2, four-phosphate adaptor protein 2; UGCG, UDP-glucose ceramide glucosyltransferase; GLTP, glycolipid transfer protein; GLTP KO, GLTP knockout.
860506p, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/860506p/product/Croda International Plc
Average 91 stars, based on 1 article reviews
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ceramide  (Croda International Plc)
98
Croda International Plc ceramide
Cells do not compensate for the lack of GLTP by increasing levels of <t>UGCG</t> or LTPs, although protein localizations are altered in the absence of GLTP . A , Western blot of analysis of the levels of UGCG, FAPP2 and CERT in WT HeLa, GLTP KO, GLTP rescue and ΔFFAT-GLTP HeLa cells. Antibodies used are described in the Materials section. Beta-actin was used as a loading control. The protein band in the upper blot that runs between FAPP2, and beta-actin is unidentified. B , representative fluorescence images of coimmunostaining of glucosylceramide synthase UGCG ( green ) and Golgi marker golgin-97 ( red ) in HeLa WT cells and, ( C ) GLTP KO HeLa cells. D , immunostaining of UGCG ( green ) and golgin-97 ( red ) in GLTP rescue HeLa cells and ( E ) ΔFFAT-GLTP expressing HeLa cells. The scale bar represents 200 μm. The subpanels are higher magnifications (2x) of representative cells. CERT, ceramide transport protein; FAPP2, four-phosphate adaptor protein 2; UGCG, UDP-glucose ceramide glucosyltransferase; GLTP, glycolipid transfer protein; GLTP KO, GLTP knockout.
Ceramide, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ceramide - by Bioz Stars, 2026-05
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ceramide sphingoid internal standard mixture  (Croda International Plc)
95
Croda International Plc ceramide sphingoid internal standard mixture
Cells do not compensate for the lack of GLTP by increasing levels of <t>UGCG</t> or LTPs, although protein localizations are altered in the absence of GLTP . A , Western blot of analysis of the levels of UGCG, FAPP2 and CERT in WT HeLa, GLTP KO, GLTP rescue and ΔFFAT-GLTP HeLa cells. Antibodies used are described in the Materials section. Beta-actin was used as a loading control. The protein band in the upper blot that runs between FAPP2, and beta-actin is unidentified. B , representative fluorescence images of coimmunostaining of glucosylceramide synthase UGCG ( green ) and Golgi marker golgin-97 ( red ) in HeLa WT cells and, ( C ) GLTP KO HeLa cells. D , immunostaining of UGCG ( green ) and golgin-97 ( red ) in GLTP rescue HeLa cells and ( E ) ΔFFAT-GLTP expressing HeLa cells. The scale bar represents 200 μm. The subpanels are higher magnifications (2x) of representative cells. CERT, ceramide transport protein; FAPP2, four-phosphate adaptor protein 2; UGCG, UDP-glucose ceramide glucosyltransferase; GLTP, glycolipid transfer protein; GLTP KO, GLTP knockout.
Ceramide Sphingoid Internal Standard Mixture, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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⍺ galactosyl ceramide  (Croda International Plc)
98
Croda International Plc ⍺ galactosyl ceramide
Extracted B. burgdorferi lipids (∼0.7-0.8 nmol total) were spotted on nitrocellulose films, along with spots of palmitoyl oleoyl phosphatidylcholine (POPC) (39 nmol), cholesteryl stearate (47 nmol), 1,2-dipalmitoyl-sn-glycerol (DAG) (80 nmol), <t>and</t> <t>⍺-galactosyl</t> <t>ceramide</t> (21 nmol). After blocking with bovine serum albumin, films were equilibrated with TULIP2, washed, developed with mouse anti-TULIP2 antibody and horseradish peroxidase-linked anti-mouse IgG, and imaged by chemiluminescence. Spot intensities, quantified by ImageJ, were normalized to B. burgdorferi lipids. Statistical significance (t-test): **, P < 0.005; ***, P< 0.0005; n.s., not significant
⍺ Galactosyl Ceramide, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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internal standards c17 ceramide  (Croda International Plc)
96
Croda International Plc internal standards c17 ceramide
Extracted B. burgdorferi lipids (∼0.7-0.8 nmol total) were spotted on nitrocellulose films, along with spots of palmitoyl oleoyl phosphatidylcholine (POPC) (39 nmol), cholesteryl stearate (47 nmol), 1,2-dipalmitoyl-sn-glycerol (DAG) (80 nmol), <t>and</t> <t>⍺-galactosyl</t> <t>ceramide</t> (21 nmol). After blocking with bovine serum albumin, films were equilibrated with TULIP2, washed, developed with mouse anti-TULIP2 antibody and horseradish peroxidase-linked anti-mouse IgG, and imaged by chemiluminescence. Spot intensities, quantified by ImageJ, were normalized to B. burgdorferi lipids. Statistical significance (t-test): **, P < 0.005; ***, P< 0.0005; n.s., not significant
Internal Standards C17 Ceramide, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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internal standards c17 ceramide - by Bioz Stars, 2026-05
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n palmitoyl d31 d erythro sphingosine  (Croda International Plc)
94
Croda International Plc n palmitoyl d31 d erythro sphingosine
Extracted B. burgdorferi lipids (∼0.7-0.8 nmol total) were spotted on nitrocellulose films, along with spots of palmitoyl oleoyl phosphatidylcholine (POPC) (39 nmol), cholesteryl stearate (47 nmol), 1,2-dipalmitoyl-sn-glycerol (DAG) (80 nmol), <t>and</t> <t>⍺-galactosyl</t> <t>ceramide</t> (21 nmol). After blocking with bovine serum albumin, films were equilibrated with TULIP2, washed, developed with mouse anti-TULIP2 antibody and horseradish peroxidase-linked anti-mouse IgG, and imaged by chemiluminescence. Spot intensities, quantified by ImageJ, were normalized to B. burgdorferi lipids. Statistical significance (t-test): **, P < 0.005; ***, P< 0.0005; n.s., not significant
N Palmitoyl D31 D Erythro Sphingosine, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
n palmitoyl d31 d erythro sphingosine - by Bioz Stars, 2026-05
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internal standard mix  (Croda International Plc)
94
Croda International Plc internal standard mix
Extracted B. burgdorferi lipids (∼0.7-0.8 nmol total) were spotted on nitrocellulose films, along with spots of palmitoyl oleoyl phosphatidylcholine (POPC) (39 nmol), cholesteryl stearate (47 nmol), 1,2-dipalmitoyl-sn-glycerol (DAG) (80 nmol), <t>and</t> <t>⍺-galactosyl</t> <t>ceramide</t> (21 nmol). After blocking with bovine serum albumin, films were equilibrated with TULIP2, washed, developed with mouse anti-TULIP2 antibody and horseradish peroxidase-linked anti-mouse IgG, and imaged by chemiluminescence. Spot intensities, quantified by ImageJ, were normalized to B. burgdorferi lipids. Statistical significance (t-test): **, P < 0.005; ***, P< 0.0005; n.s., not significant
Internal Standard Mix, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/internal standard mix/product/Croda International Plc
Average 94 stars, based on 1 article reviews
internal standard mix - by Bioz Stars, 2026-05
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pentadecanoic d29 acid  (Croda International Plc)
94
Croda International Plc pentadecanoic d29 acid
Extracted B. burgdorferi lipids (∼0.7-0.8 nmol total) were spotted on nitrocellulose films, along with spots of palmitoyl oleoyl phosphatidylcholine (POPC) (39 nmol), cholesteryl stearate (47 nmol), 1,2-dipalmitoyl-sn-glycerol (DAG) (80 nmol), <t>and</t> <t>⍺-galactosyl</t> <t>ceramide</t> (21 nmol). After blocking with bovine serum albumin, films were equilibrated with TULIP2, washed, developed with mouse anti-TULIP2 antibody and horseradish peroxidase-linked anti-mouse IgG, and imaged by chemiluminescence. Spot intensities, quantified by ImageJ, were normalized to B. burgdorferi lipids. Statistical significance (t-test): **, P < 0.005; ***, P< 0.0005; n.s., not significant
Pentadecanoic D29 Acid, supplied by Croda International Plc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pentadecanoic d29 acid/product/Croda International Plc
Average 94 stars, based on 1 article reviews
pentadecanoic d29 acid - by Bioz Stars, 2026-05
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Image Search Results


Cells do not compensate for the lack of GLTP by increasing levels of UGCG or LTPs, although protein localizations are altered in the absence of GLTP . A , Western blot of analysis of the levels of UGCG, FAPP2 and CERT in WT HeLa, GLTP KO, GLTP rescue and ΔFFAT-GLTP HeLa cells. Antibodies used are described in the Materials section. Beta-actin was used as a loading control. The protein band in the upper blot that runs between FAPP2, and beta-actin is unidentified. B , representative fluorescence images of coimmunostaining of glucosylceramide synthase UGCG ( green ) and Golgi marker golgin-97 ( red ) in HeLa WT cells and, ( C ) GLTP KO HeLa cells. D , immunostaining of UGCG ( green ) and golgin-97 ( red ) in GLTP rescue HeLa cells and ( E ) ΔFFAT-GLTP expressing HeLa cells. The scale bar represents 200 μm. The subpanels are higher magnifications (2x) of representative cells. CERT, ceramide transport protein; FAPP2, four-phosphate adaptor protein 2; UGCG, UDP-glucose ceramide glucosyltransferase; GLTP, glycolipid transfer protein; GLTP KO, GLTP knockout.

Journal: The Journal of Biological Chemistry

Article Title: Glycolipid transfer protein modulates vesicular trafficking from the endoplasmic reticulum in HeLa cells

doi: 10.1016/j.jbc.2026.111378

Figure Lengend Snippet: Cells do not compensate for the lack of GLTP by increasing levels of UGCG or LTPs, although protein localizations are altered in the absence of GLTP . A , Western blot of analysis of the levels of UGCG, FAPP2 and CERT in WT HeLa, GLTP KO, GLTP rescue and ΔFFAT-GLTP HeLa cells. Antibodies used are described in the Materials section. Beta-actin was used as a loading control. The protein band in the upper blot that runs between FAPP2, and beta-actin is unidentified. B , representative fluorescence images of coimmunostaining of glucosylceramide synthase UGCG ( green ) and Golgi marker golgin-97 ( red ) in HeLa WT cells and, ( C ) GLTP KO HeLa cells. D , immunostaining of UGCG ( green ) and golgin-97 ( red ) in GLTP rescue HeLa cells and ( E ) ΔFFAT-GLTP expressing HeLa cells. The scale bar represents 200 μm. The subpanels are higher magnifications (2x) of representative cells. CERT, ceramide transport protein; FAPP2, four-phosphate adaptor protein 2; UGCG, UDP-glucose ceramide glucosyltransferase; GLTP, glycolipid transfer protein; GLTP KO, GLTP knockout.

Article Snippet: The primary commercial antibodies used were α-Sec12 (rabbit, AB181212 ; Abcam), α-Sec16 (rabbit, HPA005684; Sigma-Aldrich), α-Sec23 A (goat, PA5-19011; Invitrogen), α-Sec23 B (mouse, MA5-27262; Invitrogen), α-Sec31 A (rabbit, PA5-52147; Invitrogen), α-UGCG (rabbit, bs-21562R; Bioss Antibodies), α-Sar1A (rabbit, PA5-103872; Invitrogen), α-CERT (rabbit, AB151285 ; Abcam), α-FAPP2 (goat, AB38748; Abcam), α-Golgin97 (mouse, A21270; Invitrogen) α-glucosylceramide (rabbit, RAS_0010; Glycobiotech GmbH), and α-galactosylceramide (rabbit, RAS_0030; Glycobiotech GmbH).

Techniques: Western Blot, Control, Fluorescence, Marker, Immunostaining, Expressing, Knock-Out

Extracted B. burgdorferi lipids (∼0.7-0.8 nmol total) were spotted on nitrocellulose films, along with spots of palmitoyl oleoyl phosphatidylcholine (POPC) (39 nmol), cholesteryl stearate (47 nmol), 1,2-dipalmitoyl-sn-glycerol (DAG) (80 nmol), and ⍺-galactosyl ceramide (21 nmol). After blocking with bovine serum albumin, films were equilibrated with TULIP2, washed, developed with mouse anti-TULIP2 antibody and horseradish peroxidase-linked anti-mouse IgG, and imaged by chemiluminescence. Spot intensities, quantified by ImageJ, were normalized to B. burgdorferi lipids. Statistical significance (t-test): **, P < 0.005; ***, P< 0.0005; n.s., not significant

Journal: bioRxiv

Article Title: A lipid-binding protein in black-legged tick saliva selectively recognizes Borrelia burgdorferi lipids

doi: 10.64898/2026.05.04.722819

Figure Lengend Snippet: Extracted B. burgdorferi lipids (∼0.7-0.8 nmol total) were spotted on nitrocellulose films, along with spots of palmitoyl oleoyl phosphatidylcholine (POPC) (39 nmol), cholesteryl stearate (47 nmol), 1,2-dipalmitoyl-sn-glycerol (DAG) (80 nmol), and ⍺-galactosyl ceramide (21 nmol). After blocking with bovine serum albumin, films were equilibrated with TULIP2, washed, developed with mouse anti-TULIP2 antibody and horseradish peroxidase-linked anti-mouse IgG, and imaged by chemiluminescence. Spot intensities, quantified by ImageJ, were normalized to B. burgdorferi lipids. Statistical significance (t-test): **, P < 0.005; ***, P< 0.0005; n.s., not significant

Article Snippet: Control samples for the lipid dot blots were selected to contain functional groups representative of the known composition of B. burgdorferi membrane lipids – : 1-palmitoyl-2-oleoylphosphatidylcholine (Avanti), cholesteryl stearate (Sigma), 1,2-dipalmitoylglycerol (Avanti), and ⍺-galactosyl ceramide (Avanti).

Techniques: Blocking Assay